Journal Article

An Increase in Unsaturation of Fatty Acids in Phosphatidylglycerol from Leaves Improves the Rates of Photosynthesis and Growth at Low Temperatures in Transgenic Rice Seedlings

Tohru Ariizumi, Sachie Kishitani, Rie Inatsugi, Ikuo Nishida, Norio Murata and Kinya Toriyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 7, pages 751-758
Published in print July 2002 | ISSN: 0032-0781
Published online July 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf087
An Increase in Unsaturation of Fatty Acids in Phosphatidylglycerol  from Leaves Improves the Rates of Photosynthesis and Growth at Low Temperatures in Transgenic Rice Seedlings

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The level of cis-unsaturated fatty acids in phosphatidylglycerol (PG) from rice leaves was genetically altered from 19.3% in the wild-type to 29.4 and 32.0% in T1 plants segregated with cDNAs for glycerol-3-phosphate acyltransferase of chloroplasts (GPAT; EC 2.3.1.15) from Arabidopsis (+AGPAT plant) and spinach (+SGPAT plant), respectively; and to 21.4% in a non-transformant segregated from +SGPAT plants (–SGPAT plant). In all these plants, O2 evolution from leaves was similar at 25°C and was impaired to a similar extent at 5 and 11°C. However, in parallel with the levels of cis-unsaturated fatty acids in PG, +AGPAT and +SGPAT plants showed less impaired rates of O2 evolution from leaves than the wild-type and –SGPAT plants at 14 and 17°C. In agreement with this, the fresh weight of 14-day-old seedlings increased to 571±18, 591±23, 687±32 and 705±31 mg in the wild-type, –SGPAT, +AGPAT and +SGPAT plants, respectively, after 6 weeks at 17/14°C (day/night). These results demonstrate the practical importance of the present technology with GPAT in improvement of the chilling sensitivity of crops.

Keywords: Keywords: Glycerol-3-phosphate acyltransferase — Phosphatidylglycerol — Fatty acid composition — Chilling tolerance — Oryza sativa L.; Abbreviations: ACP, acyl-carrier protein; AGPAT, GPAT from Arabidopsis; AGPAT, a cDNA for AGPAT; GPAT, glycerol-3-phosphate acyltransferase of chloroplasts; PG, phosphatidylglycerol; PVDF, poly(vinylidene difluoride); SGPAT, GPAT from spinach; SGPAT, a cDNA for SGPAT; +AGPAT plants, T1 transgenic rice with AGPAT; +SGPAT plants, T1 transgenic rice with SGPAT; –SGPAT plants, non-transgenic plants segregated from +SGPAT plants.

Journal Article.  5387 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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