Journal Article

Modulation of Proton Pumping across Proteoliposome Membranes Reconstituted with Tonoplast H<sup>+</sup>-ATPase from Cultured Rice (<i>Oryza sativa</i> L. var. Boro) Cells by Acyl Steryl Glucoside and Steryl Glucoside

Mineo Yamaguchi and Kunihiro Kasamo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 7, pages 816-822
Published in print July 2002 | ISSN: 0032-0781
Published online July 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf096
Modulation of Proton Pumping across Proteoliposome Membranes Reconstituted with Tonoplast H+-ATPase from Cultured Rice (Oryza sativa L. var. Boro) Cells by Acyl Steryl Glucoside and Steryl Glucoside

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Tonoplast H+-ATPase purified from cultured rice cells (Oryza sativa L. var. Boro) was reconstituted into asolectin liposomes containing steryl glucoside (SG) or acyl steryl glucoside (ASG), and the effects of SG and ASG on proton pumping, ATP-hydrolysis activity and proton permeability of the proteoliposome membranes were investigated. In the proteoliposomes containing 10 mol% SG, proton pumping and ATP-hydrolysis activity were increased to around 140% of those in SG-free proteoliposomes. In the proteoliposomes containing ASG, proton pumping and ATP-hydrolysis activity were decreased to one-tenth of those in ASG-free proteoliposomes at 15 mol% ASG; however, activity increased again slightly in the range between 20 and 40 mol% ASG. The change in proton pumping across the proteoliposome membrane is not due to a change of proteoliposome size nor to the location of the catalytic site of the tonoplast H+-ATPase in the proteoliposomes. SG and ASG also reduced the passive proton permeability of the proteoliposomes. These results show that SG and ASG modulate proton pumping across the tonoplast toward stimulation and depression, respectively, and they reduce the passive proton permeability of the tonoplast.

Keywords: Keywords: Acyl steryl glucoside (ASG) — Oryza sativa L.— Reconstitution — Steryl glucoside (SG) — Tonoplast H+-ATPase (EC 3.6.3.14) — Proton permeability.; Abbreviations: ASG, acyl steryl glucoside; GlcCer, glucocerebroside; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; SG, steryl glucoside.

Journal Article.  4256 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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