Journal Article

Proteomic Analysis of Leaf Peroxisomal Proteins in Greening Cotyledons of <i>Arabidopsis thaliana</i>

Youichiro Fukao, Makoto Hayashi and Mikio Nishimura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 7, pages 689-696
Published in print July 2002 | ISSN: 0032-0781
Published online July 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf101
Proteomic Analysis of Leaf Peroxisomal Proteins in Greening Cotyledons of Arabidopsis thaliana

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Leaf peroxisomes are present in greening cotyledons and contain enzymes of the glycolate pathway that functions in photorespiration. However, only a few leaf peroxisomal proteins, that is hydroxypyruvate reductase (HPR), glycolate oxidase (GO) and alanine:glyoxylate aminotransferase 1 (AGT1), have been characterized, and other functions in leaf peroxisomes have not been solved. To better understand the functions of leaf peroxisomes, we established a method to isolate leaf peroxisomes of greening cotyledons. We analyzed 53 proteins by MALDI-TOF MS and then identified 29 proteins. Among them, five proteins are related to the glycolate pathway, four proteins function in scavenging of hydrogen peroxide and additionally 20 novel leaf peroxisomal proteins were identified. In particular, protein kinases and protein phosphatase were first identified as peroxisomal proteins suggesting that protein phosphorylation is one of the regulatory mechanisms in leaf peroxisomes. Novel leaf peroxisomal proteins contained five PTS1-like proteins that have sequences where one amino acid is substituted with another one in PTS1 sequences. The PTS1 motif was suggested to have novel PTS1 sequences.

Keywords: Keywords: Arabidopsis thaliana — 2D-gel analysis — Greening cotyledon — Leaf peroxisome — Proteome.; Abbreviations: AGT1, alanine:glyoxylate aminotransferase 1; CAT, catalase; COX, cytochrome c oxidase; HPR, hydroxypyruvate reductase; IEF, isoelectrofocusing; IPG, immobilized pH gradient; GO, glycolate oxidase; MALDI-TOF MS, matrix-assisted laser desorption ionization time-of-flight mass spectroscopy; MDH, malate dehydrogenase; mPTS, membrane peroxisomal targeting signal; pAPX, ascorbate peroxidase; PMP, peroxisomal membrane protein.

Journal Article.  4910 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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