Journal Article

AtMRD1 and AtMRU1, Two Novel Genes with Altered mRNA Levels in the Methionine Over-Accumulating <i>mto1-1</i> Mutant of <i>Arabidopsis thaliana</i>

Derek B. Goto and Satoshi Naito

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 8, pages 923-931
Published in print August 2002 | ISSN: 0032-0781
Published online August 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf107
AtMRD1 and AtMRU1, Two Novel Genes with Altered mRNA Levels in the Methionine Over-Accumulating mto1-1 Mutant of Arabidopsis thaliana

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The mto1-1 mutant of Arabidopsis thaliana over-accumulates soluble methionine (Met) up to 40-fold higher than that in its Col-0 wild type. In order to identify genes regulated by altered Met concentrations, microarray analysis of gene expression in young rosettes and developing siliques of the mto1-1 mutant were performed. Expression of selected genes was then examined in detail in three developmental stages of the mto1-1 mutant using a combination of Northern hybridisation analysis and real-time PCR. Eight genes were identified that had altered mRNA accumulation levels in the mto1-1 mutant compared to that in wild-type plants. Three of the genes have known roles in plant development unrelated to amino acid biosynthesis. One other gene up-regulated specifically in mto1-1 rosettes shared similarity with the embryo-specific protein 3 (ATS3). Two novel genes, referred to as AtMRD1 and AtMRU1, were also identified that were expressed in a developmental manner in wild-type Col-0 and do not share sequence similarity with genes of known function. AtMRD1 was strongly down-regulated in both rosette and young silique tissues of the mto1-1 mutant. AtMRU1 was up-regulated approximately 3-fold in young mto1-1 rosettes and exhibited a developmental response to the mto1-1 mutation.

Keywords: Keywords: Arabidopsis thaliana — Methionine — Gene regulation — Microarray — Amino acids — Real-time PCR.; Abbreviations: aa, amino acids; CGS, cystathionine γ-synthase; DAF, days after flowering; DAI, days after imbibition; EST, expressed sequence tag; Met, methionine; ncRNA, non-coding RNA; ORF, open reading frame; RACE, rapid amplification of cDNA ends; SAM, S-adenosylmethionine.

Journal Article.  6907 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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