Journal Article

Protection of the Oxygen-Evolving Machinery by the Extrinsic Proteins of Photosystem II is Essential for Development of Cellular Thermotolerance in <i>Synechocystis</i> sp. PCC 6803

Aiko Kimura, Julian J. Eaton-Rye, Eugene H. Morita, Yoshitaka Nishiyama and Hidenori Hayashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 8, pages 932-938
Published in print August 2002 | ISSN: 0032-0781
Published online August 2002 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcf110
Protection of the Oxygen-Evolving Machinery by the Extrinsic Proteins of Photosystem II is Essential for Development of Cellular Thermotolerance in Synechocystis sp. PCC 6803

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The oxygen-evolving machinery of photosystem II in cyanobacteria is associated with three extrinsic proteins: the manganese-stabilizing protein, cytochrome c550, and PsbU. To elucidate the effect of the presence of these extrinsic proteins on the stabilization of the oxygen-evolving machinery against high-temperature stress, we inactivated the genes for these proteins individually in Synechocystis sp. PCC 6803 by targeted mutagenesis. The thermal stability of the oxygen-evolving machinery decreased in all mutated cells but the extent of the susceptibility to heat inactivation varied between the photosystems lacking the different extrinsic proteins. Cells that lacked either the manganese-stabilizing protein or cytochrome c550 were unable to enhance the thermal stability of the oxygen-evolving machinery and, moreover, failed to increase cellular thermotolerance when grown at moderately high temperatures. Our findings indicate that the three extrinsic proteins stabilize the oxygen-evolving machinery independently against high-temperature stress and that the thermal stability of the machinery influences cellular thermotolerance.

Keywords: Keywords: Extrinsic proteins — High-temperature stress — Oxygen evolution — Synechocystis — Thermotolerance.; Abbreviations: Cm, chloramphenicol; Er, erythromycin; MSP, manganese-stabilizing protein; Sp, spectinomycin.

Journal Article.  4594 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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