Journal Article

Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of <i>Atropa belladonna</i> after Treatment with Exogeneously Added Salicylic Acid

Hiroyuki Fukami, Tomiko Asakura, Hiroshi Hirano, Keiko Abe, Koichiro Shimomura and Takashi Yamakawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 9, pages 1054-1058
Published in print September 2002 | ISSN: 0032-0781
Published online September 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf119
Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid

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  • Biochemistry
  • Molecular and Cell Biology
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In Atropa belladonna hairy roots, exogeneously added salicylic acid (SA) is converted to methyl salicylate (MSA) through the reaction, which might be catalysed by S-adenosyl-l-methionine: salicylic acid carboxyl methyltransferase (SAMT). Here we cloned a cDNA for A. belladonna SAMT (AbSAMT1), which consisted of 357 aa residues. It was expressed in E. coli, and the recombinant AbSAMT1 showed SAMT activity. When A. belladonna hairy roots were exposed to a high concentration of SA, AbSAMT1 mRNA begins to be expressed 12 h after the exposure, and steady expression continued over 144 h.

Keywords: Keywords: Atropa belladonna — Salicylic acid — Methyl salicylate — S-adenosyl-l-methionine:salicylic acid carboxyl methyltransferase — O-methyltransferase.; Abbreviations: SA, salicylic acid; MSA, methyl salicylate; SAM, S-adenosyl-l-methionine; SAMT, SAM:salicylic acid carboxyl methyltransferase; OMT, O-methyltransferase; SAG, salicylic acid β-glucoside; RT-PCR, reverse transcription polymerase chain reaction; IPTG, isopropyl-β-d-thiogalactopyranoside; GC-MS, gas chromatograph-mass spectrometry;

Journal Article.  3163 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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