Journal Article

The Role of Plant CCTα in Salt- and Osmotic-Stress Tolerance

Akiyo Yamada, Mikiko Sekiguchi, Tetsuro Mimura and Yoshihiro Ozeki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 9, pages 1043-1048
Published in print September 2002 | ISSN: 0032-0781
Published online September 2002 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcf120
The Role of Plant CCTα in Salt- and Osmotic-Stress Tolerance

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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To find key genes essential for salt tolerance in the mangrove plant, Bruguiera sexangula, functional screening was performed using Escherichia coli as the host organism. A transformant expressing a cytosolic chaperonin-containing TCP-1α (CCTα) homologue displayed enhanced salt tolerance. Analysis in E. coli of the functional region revealed that a sequence of only 218 amino acids, containing the apical domain, is necessary for osmotolerance. Furthermore, this domain shows chaperone activity in vitro. Therefore, CCTα facilitates the folding of proteins without ATP or the cage-like structure, and may play an important role in stress tolerance, at least in B. sexangula.

Keywords: Keywords: Bruguiera sexangula — CCTα — Mangrove —Salt-tolerance.; Abbreviations: CCT, chaperonin containing TCP-1; TCP-1, t-complex peptide-1; CS, citrate synthase; MBP, maltose binding protein.

Journal Article.  2752 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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