Journal Article

Characterization of the ARR15 and ARR16 Response Regulators with Special Reference to the Cytokinin Signaling Pathway Mediated by the AHK4 Histidine Kinase in Roots of <i>Arabidopsis thaliana</i>

Takatoshi Kiba, Hisami Yamada and Takeshi Mizuno

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 9, pages 1059-1066
Published in print September 2002 | ISSN: 0032-0781
Published online September 2002 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcf121
Characterization of the ARR15 and ARR16 Response Regulators with Special Reference to the Cytokinin Signaling Pathway Mediated by the AHK4 Histidine Kinase in Roots of Arabidopsis thaliana

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The cytokinin receptor AHK4 histidine kinase, identified in Arabidopsis thaliana, presumably acts in concert with downstream components, such as histidine-containing phosphotransfer (HPt) factors (AHPs) and response regulators (ARRs). In this respect, we characterized a loss-of-function mutant of the AHK4 gene, named cre1-1, which showed a reduced cell number within the vascular tissues in roots. Among the 10 type-A ARR members, the expression of ARR15 and ARR16 in roots was specifically and markedly reduced in cre1-1, suggesting a link between these response regulators and the AHK4-mediated signal transduction in roots. The results for transgenic plants expressing promoter::GUS or promoter::LUC fusion genes showed that both the ARR15 and the ARR16 gene products are accumulated upon cytokinin treatment in roots. The results of GFP-fusion experiments with onion epidermal cells further showed that ARR15 was found in the nucleus, and ARR16 mainly in the cytoplasm. Together, it was suggested that ARR15 and ARR16 are distinctly implicated in the presumed AHK4-mediated signaling pathway in roots.

Keywords: Keywords: Arabidopsis — Cytokinin — Histidine kinase — Phosphorelay —Response regulators — Signal transduction.; Abbreviations: AHK, Arabidopsis histidine kinase; AHP, Arabidopsis histidine-containing phosphotransmitter; ARR, Arabidopsis response regulator.

Journal Article.  4103 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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