Journal Article

Enhanced Resistance to Salt, Cold and Wound Stresses by Overproduction of Animal Cell Death Suppressors Bcl-xL and Ced-9 in Tobacco Cells — Their Possible Contribution Through Improved Function of Organella

Jingbo Qiao, Ichiro Mitsuhara, Yosiaki Yazaki, Katsuhiro Sakano, Yoko Gotoh, Masayuki Miura and Yuko Ohashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 9, pages 992-1005
Published in print September 2002 | ISSN: 0032-0781
Published online September 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf122
Enhanced Resistance to Salt, Cold and Wound Stresses by Overproduction of Animal Cell Death Suppressors Bcl-xL and Ced-9 in Tobacco Cells — Their Possible Contribution Through Improved Function of Organella

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Overproduction of animal cell death suppressors Bcl-xL and Ced-9 conferred enhanced resistance to UV-B and paraquat treatment in tobacco plants [Mitsuhara et al. (1999) Curr. Biol. 9: 775]. We report here that the progeny could germinate in 0.2 M NaCl or at 10°C under light, while control plants could not. Suspension-cultured Bcl cells resisted NaCl treatment maintaining an active mitochondrial membrane potential for longer than control cells. When intracellular pH was determined by in vivo 31P-NMR, immediate cytoplasmic acidification by 0.3 M NaCl treatment in control cells was found to be suppressed in transgenic cells. Monitoring of cytoplasmic and vacuolar pHs in control cells indicated the vacuole was disrupted 40 min after 0.5 M NaCl treatment, while the compartment between the cytoplasm and vacuole was likely to remain intact in Bcl cells for 100 min. Enhanced shoot regeneration from cut leaf pieces and more vigorous rooting from cut stem ends were found in transgenic plants. The Bcl protein was abundant in all subcellular fractions. Based on the results in transgenic plants carrying a mutant bcl-xL gene, Bcl-xL is thought to suppress cell death and enhance the viability of plants in stressful environments by contributing to the maintenance of the homeostasis of organella.

Keywords: Keywords: Bcl-xL — Ced-9 — Cell death — Cold-stress — Mitochondrial active potential — Salt tolerance — Vacuole.; Abbreviations: APX, ascorbic acid peroxidase; GS, glutamine synthase; Luc, luciferase; MDP, methylene diphosphate; PCR, polymerase chain reaction; PI, propidium iodide; Rh123, rhodamine 123; ROS, reactive oxygen species; SOD, superoxide dismutase; VDAC, voltage-dependent anion channel.

Journal Article.  8059 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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