Journal Article

The Cyanobacterial PilT Protein Responsible for Cell Motility and Transformation Hydrolyzes ATP

Shinobu Okamoto and Masayuki Ohmori

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 10, pages 1127-1136
Published in print October 2002 | ISSN: 0032-0781
Published online October 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf128
The Cyanobacterial PilT Protein Responsible for Cell Motility and Transformation Hydrolyzes ATP

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The unicellular cyanobacterium, Synechocystis sp. PCC 6803 is motile. A homologue of the PilT protein family, required for twitching motility in Pseudomonas aeruginosa and social gliding motility in Myxococcus xanthus, was found to be necessarily associated with cyanobacterial motility. The pilT1 (slr0161) mutant shows a pleotropic phenotype, defects in individual cell motility, and an increased number of long surface pili. Furthermore, the mutant loses its ability of natural competency. These findings demonstrate that PilT1 is essential for both cell motility and competency. Since the pilT gene contains a consensus ATP-binding motif (Walker boxes), the PilT protein is suggested for supplying energy for cell motility. The product of pilT1, overproduced in Escherichia coli and purified by Ni-affinity chromatography, hydrolyzes ATP in vitro.

Keywords: Keywords: ATPase — Cyanobacteria — Motility — Pili — pilT.; Abbreviations: IPTG, isopropyl-β-d-(–)-thiogalactopyranoside; PMSF, phenylmethanesulfonyl fluoride.

Journal Article.  5986 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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