Journal Article

The Transcript-Level-Independent Activation of Ornithine Decarboxylase in Suspension-Cultured BY2 Cells Entering the Cell Cycle

Su-Hwan Kwak and Sun Hi Lee

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 10, pages 1165-1170
Published in print October 2002 | ISSN: 0032-0781
Published online October 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf132
The Transcript-Level-Independent Activation of Ornithine Decarboxylase in Suspension-Cultured BY2 Cells Entering the Cell Cycle

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The regulation of ornithine decarboxylase (ODC) expression was studied in suspension-cultured tobacco (Nicotiana tabacum L.) BY2 cells. ODC activity increased rapidly 3 h after cells re-entered the cell cycle from the stationary phase, corresponding to the G1 phase, and continued to increase in the subsequent S phase, while the ODC transcript level increased only transiently. ODC activity was suppressed by sucrose-deficiency, while the ODC transcript level was not affected. U0126, a specific inhibitor of mammalian MAPK kinases (MEKs), significantly reduced ODC enzyme activity, but not the ODC transcript level. These results suggest that ODC activity is regulated independently of its transcript level in BY2 cells, and that sucrose and a U0126-sensitive protein kinase are required for the transcript-level-independent activation of ODC.

Keywords: Keywords: BY2 — Cell cycle — MAPK kinase — Nicotiana tabacum — Ornithine decarboxylase — Sucrose — U0126.; Abbreviations: DTT, dithiothreitol; MAPK, mitogen-activated protein kinase; MEK, MAPK kinase; ODC, ornithine decarboxylase; uORF, upstream open reading frame; UTR, untranslated region.

Journal Article.  3951 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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