Journal Article

Promotion of Transcript Accumulation of Novel <i>Zinnia</i> Immature Xylem-Specific HD-Zip III Homeobox Genes by Brassinosteroids

Kyoko Ohashi-Ito, Taku Demura and Hiroo Fukuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 10, pages 1146-1153
Published in print October 2002 | ISSN: 0032-0781
Published online October 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf135
Promotion of Transcript Accumulation of Novel Zinnia Immature Xylem-Specific HD-Zip III Homeobox Genes by Brassinosteroids

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We isolated three novel homeobox genes (ZeHB-10, -11 and -12) from Zinnia elegans to elucidate the molecular mechanism underlying vascular system formation. ZeHB-10, -11 and -12 encode for HD-Zip proteins of the class III to which Arabidopsis Athb-8, -9, -14, -15 and IFL1 belong. In situ hybridization analysis demonstrated that the ZeHB-10, -11 and -12 mRNAs accumulated preferentially in procambium and immature xylem cells in 14-day-old plants. Transcripts for the three genes also accumulated in cultured Zinnia cells in a xylogenesis-specific manner. The accumulation of transcripts for all of ZeHB-10, -11 and -12 in cultured Zinnia cells was suppressed strongly by uniconazole, an inhibitor of brassinosteroid synthesis, and such suppression was reversed by the addition of brassinolide, a biologically active brassinosteroid. Thus the expression of ZeHB-10, -11 and -12 may be regulated by endogenous levels of brassinosteroids. Taken together with the fact that ZeHB-10, -11 and -12 proteins can bind to each other in yeast, the roles of HD-Zip III genes in vascular development are discussed.

Keywords: Keywords: Brassinosteroid — Dimerization — HD-Zip homeobox genes — Vascular bundle — Xylem differentiation — Zinnia elegans.; Abbreviations: BA, 6-benzyladenine; DIG, digoxigenin; NAA, 1-naphthalenacetic acid.

Journal Article.  5191 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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