Journal Article

Zinc Ions Inhibit the Protein–DNA Complex Formation between Cyanobacterial Transcription Factor SmtB and its Recognition DNA Sequences

Eugene Hayato Morita, Miki Wakamatsu, Koichi Uegaki, Noboru Yumoto, Yoshimasa Kyogoku and Hidenori Hayashi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 10, pages 1254-1258
Published in print October 2002 | ISSN: 0032-0781
Published online October 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf140
Zinc Ions Inhibit the Protein–DNA Complex Formation between Cyanobacterial Transcription Factor SmtB and its Recognition DNA Sequences

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  • Biochemistry
  • Molecular and Cell Biology
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SmtB is a trans-acting dimeric repressor of the metal-regulated smtA gene, and the release of SmtB from the smtA operator/promoter region is essential for the tolerance to Zn2+ stress by SmtA expression. Gel retardation assaying demonstrated that different sizes of SmtB–DNA complexes were formed depending on the DNA sequences, and the amounts of these complexes decreased in the presence of Zn2+. Here, we present the first direct evidence that Zn2+ (>4 µM) inhibits the SmtB–DNA complex formation in vitro, which ensures the physiological functions of SmtB as a Zn2+ sensor and a transcription factor.

Keywords: Keywords: Cyanobacteria — Heavy-metal ion sensor — Operator/promoter region — Transcription factor.; Abbreviation: Bbs, SmtB binding sequence.

Journal Article.  2724 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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