Journal Article

Cloning, Functional Expression, and Mutational Analysis of a cDNA for <i>Lotus japonicus</i> Mitochondrial Phosphate Transporter

Kazuki Nakamori, Reona Takabatake, Yosuke Umehara, Hiroshi Kouchi, Katsura Izui and Shingo Hata

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 10, pages 1250-1253
Published in print October 2002 | ISSN: 0032-0781
Published online October 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf141
Cloning, Functional Expression, and Mutational Analysis of a cDNA for Lotus japonicus Mitochondrial Phosphate Transporter

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

A full-length cDNA encoding mitochondrial phosphate transporter was isolated from symbiotic nodules of Lotus japonicus, a model legume. The gene appeared to be single-copied and its transcript was abundant in roots, symbiotic root nodules and shoots, in that order. The L. japonicus transporter has a conserved N-ethylmaleimide-reactive Cys residue. Site-directed mutagenesis and phosphate transport assaying in vitro showed that this Cys residue is preferable for the transport activity, although it can be replaced to some extent by Ser, but not by Ala.

Keywords: Keywords: Lotus japonicus — N-ethylmaleimide — Proteoliposomes — Recombinant protein — Site-directed mutagenesis.; Abbreviations: MPT, mitochondrial phosphate transporter; NEM, N-ethylmaleimide; Pi, inorganic phosphate.

Journal Article.  1879 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.