Journal Article

Expression of the 12-Oxophytodienoic Acid 10,11-Reductase Gene in the Compatible Interaction between Pea and Fungal Pathogen

Yasuhiro Ishiga, Akiko Funato, Tomoyuki Tachiki, Kazuhiro Toyoda, Tomonori Shiraishi, Tetsuji Yamada and Yuki Ichinose

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 10, pages 1210-1220
Published in print October 2002 | ISSN: 0032-0781
Published online October 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf144
Expression of the 12-Oxophytodienoic Acid 10,11-Reductase Gene in the Compatible Interaction between Pea and Fungal Pathogen

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Suppressors produced by Mycosphaerella pinodes are glycopeptides to block pea defense responses induced by elicitors. A clone, S64, was isolated as cDNA for suppressor-inducible gene from pea epicotyls. The treatment of pea epicotyls with suppressor alone induced an increase of S64 mRNA within 1 h, and it reached a maximum level at 3 h after treatment. The induction was not affected by application of the elicitor, indicating that the suppressor has a dominant action to regulate S64 gene expression. S64 was also induced by inoculation with a virulent pathogen, M. pinodes, but not by inoculation with a non-pathogen, Ascochyta rabiei, nor by treatment with fungal elicitor. The deduced structure of S64 showed high homology to 12-oxophytodienoic acid reductase (OPR) in Arabidopsis thaliana. A recombinant protein derived from S64 had OPR activity, suggesting compatibility-specific activation of the octadecanoid pathway in plants. Treatment with jasmonic acid (JA) or methyl jasmonic acid, end products of the octadecanoid pathway, inhibited the elicitor-induced accumulation of PAL mRNA in pea. These results indicate that the suppressor-induced S64 gene expression leads to the production of JA or related compounds, which might contribute to the establishment of compatibility by inhibiting the phenylpropanoid biosynthetic pathway.

Keywords: Keywords: Jasmonic acid — Oxophytodienoic acid reductase — Suppressor.; Abbreviations: ABA, abscisic acid; bp, base pair(s); HR, hypersensitive response; JA, jasmonic acid; MeJA, methyl jasmonic acid, OPDA, oxophytodienoic acid; OPR, OPDA reductase; ORF, open reading frame; PAL, phenylalanine ammonia-lyase; PCR, polymerase chain reaction; PI, polyphosphoinositide; SA, salicylic acid; SAR, systemic acquired resistance.

Journal Article.  7764 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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