Journal Article

Differential Tissue-Specific Response to Sulfate and Methionine of a Soybean Seed Storage Protein Promoter Region in Transgenic <i>Arabidopsis</i>

Naoko Ohkama, Derek B. Goto, Toru Fujiwara and Satoshi Naito

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 11, pages 1266-1275
Published in print November 2002 | ISSN: 0032-0781
Published online November 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf149
Differential Tissue-Specific Response to Sulfate and Methionine of a Soybean Seed Storage Protein Promoter Region in Transgenic Arabidopsis

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  • Biochemistry
  • Molecular and Cell Biology
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Expression of the gene encoding the β subunit of β-conglycinin, a major soybean seed storage protein, is upregulated by sulfur deficiency and downregulated by methionine (Met). The tissue-specificity of these regulatory mechanisms was studied using a sulfate-responsive region (βSR) from the β subunit gene promoter. Transgenic Arabidopsis thaliana lines were generated carrying a green fluorescent protein (GFP) reporter gene under control of the cauliflower mosaic virus 35S RNA promoter with a tandem repeat of the βSR element, referred to as the P35S::βSRx3: GFP transgene. Upregulation of P35S::βSRx3:GFP by sulfur deficiency was strongest in leaf margins, where symptoms of sulfur deficiency first appear. P35S::βSRx3:GFP was also upregulated at 2 d after a medium shift from sulfur-sufficient to sulfur-deficient conditions, suggesting that the chimeric promoter is an efficient indicator of sulfur nutritional status. Analysis of transgene expression in a Met-overaccumulating mto1-1 mutation background revealed that the βSR region carries sufficient information for downregulation of promoter activity by Met in developing seeds, but not in young rosettes. Comparisons with another transgenic line, in which the full-length β promoter is active in non-seed tissues, also suggested that at least two separate tissue-specific mechanisms exist for the downregulation of the β promoter by Met.

Keywords: Keywords: Arabidopsis thaliana — β subunit of β-conglycinin — GFP reporter gene — Methionine — mto1-1 mutation — Sulfur deficiency.; Abbreviations: CaMV, cauliflower mosaic virus; DAI, days after imbitition; DAS, days after shift; γ-EC, γ-glutamylcysteine; GSH, glutathione; GFP, green fluorescent protein; OAS, O-acetyl-l-serine; ΔS, sulfate-deficient.

Journal Article.  7048 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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