Journal Article

A Sensitive and Rapid Bioassay of Homogalacturonan Synthase Using 2-Aminobenzamide-Labeled Oligogalacturonides

Tadashi Ishii

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 11, pages 1386-1389
Published in print November 2002 | ISSN: 0032-0781
Published online November 2002 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcf150
A Sensitive and Rapid Bioassay of Homogalacturonan Synthase Using  2-Aminobenzamide-Labeled Oligogalacturonides

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  • Biochemistry
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Polygalacturonate 4-α-galacturonosyltransferase (GalA T) activity was detected in the microsomal fraction isolated from pumpkin (Cucurbia moschata Duchesne, cv. Tokyou-Kabocha) seedlings using UDP-GalA and 2-aminobenzamide (2AB)-labeled oligogalacturonides. A 2AB-labeled undecagalacturonide was elongated by the attachment of galacturonic acid (GalA) residues to give 2AB-labeled oligogalacturonides with a degree of polymerization (DP) beetween 12 and 17. Exogenous 2AB-labeled oligogalacturonide acceptors with a DP >3 are effective acceptor molecules for pumpkin GalA T.

Keywords: Keywords: 2-Aminobenzamide-labeled oligogalacturonide — Cucurbia (etiolated epicotyls) —Fluorescence detection — Galacturonosyltransferase — Homogalacturonan — Pectin.; Abbreviations: 2AB, 2-aminobenzamide; DP, degree of polymerization; EPG, endo-polygalacturonase; GalA, galacturonic acid; GalA T, polygalacturonate 4-α-galacturonosyltransferase; HPAEC, high-performance anion exchange chromatography.

Journal Article.  2713 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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