Journal Article

The Degradation of the Large Subunit of Ribulose-1,5-bisphosphate Carboxylase/oxygenase into the 44-kDa Fragment in the Lysates of Chloroplasts Incubated in Darkness

Norimoto Kokubun, Hiroyuki Ishida, Amane Makino and Tadahiko Mae

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 11, pages 1390-1395
Published in print November 2002 | ISSN: 0032-0781
Published online November 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf159
The Degradation of the Large Subunit of Ribulose-1,5-bisphosphate Carboxylase/oxygenase into the 44-kDa Fragment in the Lysates of Chloroplasts Incubated in Darkness

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Lysates of chloroplasts isolated from naturally senescing wheat leaves were incubated in darkness. The 44-kDa fragment, lacking the N-terminal-side portion of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (LSU), was found by immunoblotting with the LSU site-specific antibodies. Analysis of its N-terminal amino acid sequence indicated that the LSU was specifically cleaved at the peptide bond between Phe-40 and Arg-41. The site was located on the surface of the molecule and faced outward. Such cleavage of the LSU has not been previously reported. It is indicated that the cleavage was triggered by an unknown protease existing in chloroplasts.

Keywords: Keywords: Chloroplast — Protease — Protein degradation — Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) — Wheat (Triticum aestivum).; Abbreviations: anti-LSU-N or anti-LSU-C, site-specific antibodies against N-terminal or C-terminal portion of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase; LSU, large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase; Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase.

Journal Article.  3306 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.