Journal Article

Transcriptional Response of Soybean Suspension-cultured Cells Induced by Nod Factors Obtained from <i>Bradyrhizobium japonicum</i> USDA110

Tsuneo Hakoyama, Tadashi Yokoyama, Hiroshi Kouchi, Ken-ichi Tsuchiya, Hisatoshi Kaku and Yasuhiro Arima

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 11, pages 1314-1322
Published in print November 2002 | ISSN: 0032-0781
Published online November 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf160
Transcriptional Response of Soybean Suspension-cultured Cells Induced by Nod Factors Obtained from Bradyrhizobium japonicum USDA110

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  • Biochemistry
  • Molecular and Cell Biology
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Genes responding to Nod factors were picked up by the application of a differential display method for soybean suspension-cultured cells. Forty-five cDNA fragments derived from such genes were detected. Seven fragments (ssc1–ssc7) were successfully cloned. The putative product of genes corresponding to ssc1 was estimated to be a disease-resistance protein relating to the induction of the plant defense response against pathogens, and that corresponding to ssc7 was a sucrose transporter. Amino acid sequences deduced from full-length cDNA corresponding to ssc2 and ssc4 were investigated, and it was shown that these polypeptides were equipped with a leucine zipper motif and with phosphorylation sites that were targeted by tyrosin kinase and cAMP-dependent protein kinase, respectively. In a differential display experiment, the transcriptional levels of three genes corresponding to ssc2, ssc3 and ssc5 were estimated to be up-regulated at 6 h after initiation of the treatment and the remaining four were estimated to be down-regulated. However, transcription of the genes corresponding to all ssc was clearly repressed within 2 h after initiation of the treatment. Five of them were restored to their transcriptional level 6 h after initiation of the treatment, although the others were repressed throughout the experimental period.

Keywords: Keywords: Bradyrhizobium — Differential display — Down regulation — Nod factors — Soybean — Suspension-cultured cells.; Abbreviation: ssc, soybean suspension culture clone.

Journal Article.  5146 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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