Journal Article

Characterization of Polypeptides Accumulated in the Latex Cytosol of Rubber Trees Affected by the Tapping Panel Dryness Syndrome

Unchera Sookmark, Valérie Pujade-Renaud, Hervé Chrestin, Régis Lacote, Chinda Naiyanetr, Marc Seguin, Phayao Romruensukharom and Jarunya Narangajavana

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 11, pages 1323-1333
Published in print November 2002 | ISSN: 0032-0781
Published online November 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf161
Characterization of Polypeptides Accumulated in the Latex Cytosol of Rubber Trees Affected by the Tapping Panel Dryness Syndrome

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  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The tapping panel dryness (TPD) syndrome of rubber is characterized by the reduction or ultimately total cessation of latex flow upon tapping, due to physiological disorders in the bark tissue. The protein pattern in the cytoplasm from healthy and TPD tree latex cells was compared by electrophoresis. Two polypeptides (P15 and P22) of 15 and 22 kDa, respectively, were found to accumulate in the cytosol of the TPD-affected trees, whereas a 29 kDa polypeptide (P29) appeared de novo. P15 and P22 were identified as REF (Hev b1) and SRPP (Hev b3), respectively, two proteins proposed to be involved in rubber biosynthesis. P29 appeared to be a new member of the patatin-like protein family. Specific molecular probes were designed for a detailed characterization of REF and SRPP gene expression and RFLP mapping. This allowed the demonstration that REF and SRPP display very similar expression profiles. They are highly over-expressed by the tapping-induced metabolic activation, although not by wounding per se, or ethylene or ABA. In addition to this similarity in gene expression, they were found to share one common locus in the genome. No significant difference in REF and SRPP gene expression was observed between healthy and TPD trees, indicating that their TPD-related accumulation in the cytosol was not transcriptionally regulated. Western blot analysis demonstrated that osmotic lysis of the sedimentable organelles (lutoids) in vitro caused the release of REF and SRPP from the rubber particle membrane into the cytosol. A mechanism of cellular delocalization as a consequence of the lutoids instability is proposed to explain REF and SRPP accumulation in the cytosol of TPD trees.

Keywords: Keywords: Cellular decompartmentalization — Ethylene — Hevea brasiliensis — Latex — Rubber tree — Tapping panel dryness (TPD).; The first two authors contributed equally to the work.

Journal Article.  7458 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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