Journal Article

Expression of Symbiotic and Nonsymbiotic Globin Genes Responding to Microsymbionts on <i>Lotus japonicus</i>

Toshiki Uchiumi, Yoshikazu Shimoda, Tomoko Tsuruta, Yumi Mukoyoshi, Akihiro Suzuki, Keishi Senoo, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Shiro Higashi and Mikiko Abe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 11, pages 1351-1358
Published in print November 2002 | ISSN: 0032-0781
Published online November 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf165
Expression of Symbiotic and Nonsymbiotic Globin Genes Responding to Microsymbionts on Lotus japonicus

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Leguminous plants have both symbiotic and nonsymbiotic hemoglobin (sym- and nonsym-Hb) genes. Three symbiotic (LjLb1, 2, 3) and one nonsymbiotic (LjNSG1) Hb genes were isolated from a genomic library of Lotus japonicus MG20 Miyakojima. Two motif sequences (AAAGAT and CTCTT) critical for nodule specific expression were conserved on the 5′-upstream sequence of LjLb1, 2 and 3. The 5′-upstream region of LjNSG1 contained the sequence consensus for nonsym-Hb. RT-PCR with specific primer sets for each LjLb gene showed that all the sym-Hb genes (LjLb1, 2, 3) were expressed specifically and strongly in root nodules. The expression of LjLb1, 2 and 3 could not be detected in root, leaf or stem of a mature plant, whereas low level expression was detected in seedlings by RT-PCR. This suggests that sym-Hbs may have another unknown function besides being oxygen transporter for the microsymbiont in symbiotic nitrogen fixation. The expression of LjNSG1, examined with RT-PCR, was detected at low level in root, leaf and stem. The expression of LjNSG1 was enhanced in root nodules, whereas it was repressed in roots colonized by mycorrhizal fungi Glomus sp. R10. The repression of the nonsym-Hb gene was also observed in the roots of Medicago sativa colonized by Glomus sp. R10.

Keywords: Keywords: Expression — Hemoglobin — Lotus japonicus — Mycorrhiza — Nodule.; Abbreviations: AM, arbuscular mycorrhiza; Hb, hemoglobin; Lb, leghemoglobin; nonsym-Hb, nonsymbiotic hemoglobin; sym-Hb, symbiotic hemoglobin.

Journal Article.  5633 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.