Journal Article

Phosphatidylcholine Biosynthesis at Low Temperature: Differential Expression of CTP:Phosphorylcholine Cytidylyltransferase Isogenes in <i>Arabidopsis thaliana</i>

Rie Inatsugi, Masanobu Nakamura and Ikuo Nishida

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 11, pages 1342-1350
Published in print November 2002 | ISSN: 0032-0781
Published online November 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf169
Phosphatidylcholine Biosynthesis at Low Temperature: Differential Expression of CTP:Phosphorylcholine Cytidylyltransferase Isogenes in Arabidopsis thaliana

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We cloned the gene and a cDNA for a second CTP: phosphorylcholine cytidylyltransferase (CCT, EC 2.7.7.15) annotated in chromosome 4 by the Arabidopsis genome project, and designated the gene AtCCT2 to discriminate it from the isogene AtCCT1 in chromosome 2. When Arabidopsis plants were chilled at 2°C for 12 h, the level of AtCCT2 transcripts in the rosettes increased about 6-fold over that before 2°C treatment. By contrast, no significant change occurred in the level of AtCCT1 transcripts during 7 d of 2°C treatment. Immunoblot analysis revealed that the level of AtCCT2 in the rosettes chilled at 2°C increased, and that the level of AtCCT1 showed minor changes, when compared with those before cold treatment. Total CCT activity measured at 2°C increased in plants subjected to 2°C treatment, and this increase was sufficient to account for lipid changes induced by the 2°C treatment. We therefore concluded that Arabidopsis utilizes two distinct CCT isozymes for CDP-choline synthesis during cold acclimation. Our findings are important in understanding the physiological functions of CCT isozymes in Arabidopsis and will also stimulate efforts to understand the physiological significance of phosphatidylcholine at low temperatures.

Keywords: Keywords: Arabidopsis — Cold acclimation — CRT/DRE motif — CTP:phosphorylcholine cytidylyltransferase (EC 2.7.7.15) — Phosphatidylcholine.; Abbreviations: CCT, CTP:phosphorylcholine cytidylyltransferase; COR, cold-responsive genes; CRT/DRE, C-repeat/dehydration responsive element; DGDG, digalactosyldiacylglycerol; DTT, dithiothreitol; EGTA, ethylene glycol bis(2-aminoethyl ether)tetraacetic acid; MGDG, monogalactosyldiacylglycerol; MOPS, 3-(N-morpholino)propanesulfonic acid; PC, phosphatidylcholine; RT-PCR, reverse transcription-PCR; SQDG, sulfoquinovosyldiacylglycerol; PMSF, phenylmethanesulfonyl fluoride.

Journal Article.  6198 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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