Journal Article

QTL Analysis of Al Tolerance in Recombinant Inbred Lines of <i>Arabidopsis thaliana</i>

Yuriko Kobayashi and Hiroyuki Koyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 12, pages 1526-1533
Published in print December 2002 | ISSN: 0032-0781
Published online December 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf174
QTL Analysis of Al Tolerance in Recombinant Inbred Lines of Arabidopsis thaliana

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Quantitative trait loci (QTLs) and epistasis for Arabidopsis thaliana aluminum (Al) tolerance were analyzed using a recombinant inbred (RI) population of 100 lines derived from a cross between Landsberg erecta and Columbia (Col). Root growth of the RI population was determined in hydroponics using solutions containing 0 or 4 µM of AlCl3 and a series of nutrients, except Pi, at pH 5.0. Al tolerance was defined as relative root length [RRL: plus Al/minus Al (%)], and the RI lines ranged from 22.6 to 97.4% with a broad sense heritability of 0.99. Using the composite interval mapping method, two significant single factor QTLs (P<0.05) were detected by RRL on chromosomes 1 and 4, where the Col allele showed positive and negative effects on the Al tolerance. These QTLs could explain about 43% of the total variation of Al tolerance among the RI population. On the other hand, five epistatic loci pairs were identified by the complete pair-wise search method (P<0.0005). No single factor QTL and epistatic loci pairs were shared by the root length in the control and the RRL, suggesting that the loci identified by the RRL would be specific for Al treatment and controlling Al tolerance among the RI population.

Keywords: Keywords: Aluminum tolerance — Arabidopsis thaliana — Epistasis — Hydroponic culture — QTL.; Abbreviations: CIM, composite interval mapping; Col, Columbia; Ler, Landsberg erecta; RI, recombinant inbred; RRL, relative root length; QTL, quantitative traits loci.

Journal Article.  5805 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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