Journal Article

Electrical Perception of “Death Message” in <i>Chara</i>: Analysis of Rapid Component and Ionic Process

Teruo Shimmen

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 12, pages 1575-1584
Published in print December 2002 | ISSN: 0032-0781
Published online December 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf182
Electrical Perception of “Death Message” in Chara: Analysis of Rapid Component and Ionic Process

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Electrical response upon wounding was analyzed in Chara corallina. A specimen comprising two adjoining internodal cells was prepared. One cell (victim cell) was killed by cutting and any changes in the membrane potential of the neighboring cell (the receptor cell) were measured. Upon cutting the victim cell, the receptor cell generated four kinds of depolarizations: (1) rapid component, (2) slow and long-lasting component, (3) action potential and (4) small spike. Rapid and slow components were observed in most cells. On the other hand, the action potential and small spike were not always ubiquitous among specimens. When an action potential was generated just after cutting the victim cell, the rapid component could not be observed due to masking by the action potential. It was suggested that both rapid and slow components were generated at the nodal end. On the other hand, action potentials were thought to be generated at the flank of the receptor cell. High turgor pressure of the cell was necessary for generating both rapid and slow components. Experiments under K+-induced depolarization unequivocally showed that the Cl channel at the nodal end of the receptor cell was activated upon cutting the victim cell.

Keywords: Keywords: Chara — Chloride channel — Death message — Membrane potential — Wounding response.; Abbreviations: APW, artificial pond water; [Ca2+], concentration of free Ca2+ in external medium; [Cl–], concentration of Cl– in external medium; ΔErap, amplitude of rapid component; ΔEsl, amplitude of slow component; EA, electrode in pool A; EAB, potential difference between pools A and B; EB, electrode in pool B; EBC, potential difference between pools B and C; EC, electrode in pool C; Em, membrane potential; (Em)r, resting membrane potential; (Em)rap, peak potential of rapid component; (Em)sl, peak potential of slow component; LB, length of portion of receptor cell in pool B; Lc, length of portion of receptor cell in pool C; R cell, receptor cell; V cell, victim cell.

Journal Article.  7201 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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