Journal Article

The NADPH:Quinone Oxidoreductase P1-ζ-crystallin in <i>Arabidopsis</i> Catalyzes the <i>α</i>,<i>β</i>-Hydrogenation of 2-Alkenals: Detoxication of the Lipid Peroxide-Derived Reactive Aldehydes

Jun’ichi Mano, Yoshimitsu Torii, Shun-ichiro Hayashi, Koichi Takimoto, Kenji Matsui, Kaoru Nakamura, Dirk Inzé, Elena Babiychuk, Sergei Kushnir and Kozi Asada

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 12, pages 1445-1455
Published in print December 2002 | ISSN: 0032-0781
Published online December 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf187
The NADPH:Quinone Oxidoreductase P1-ζ-crystallin in Arabidopsis Catalyzes the α,β-Hydrogenation of 2-Alkenals: Detoxication of the Lipid Peroxide-Derived Reactive Aldehydes

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P1-ζ-crystallin (P1-ZCr) is an oxidative stress-induced NADPH:quinone oxidoreductase in Arabidopsis thaliana, but its physiological electron acceptors have not been identified. We found that recombinant P1-ZCr catalyzed the reduction of 2-alkenals of carbon chain C3-C9 with NADPH. Among these 2-alkenals, the highest specificity was observed for 4-hydroxy-(2E)-nonenal (HNE), one of the major toxic products generated from lipid peroxides. (3Z)-Hexenal and aldehydes without α,β-unsaturated bonds did not serve as electron acceptors. In the 2-alkenal molecules, P1-ZCr catalyzed the hydrogenation of α,β-unsaturated bonds, but not the reduction of the aldehyde moiety, to produce saturated aldehydes, as determined by gas chromatography/mass spectrometry. We propose the enzyme name NADPH:2-alkenal α,β-hydrogenase (ALH). A major portion of the NADPH-dependent HNE-reducing activity in A. thaliana leaves was inhibited by the specific antiserum against P1-ZCr, indicating that the endogenous P1-ZCr protein has ALH activity. Because expression of the P1-ZCr gene in A. thaliana is induced by oxidative stress treatments, we conclude that P1-ZCr functions as a defense against oxidative stress by scavenging the highly toxic, lipid peroxide-derived α,β-unsaturated aldehydes.

Keywords: Keywords: 2-Alkenal — Arabidopsis thaliana — Antioxidant — 4-Hydroxy-(2E)-hexenal — 4-Hydroxy-(2E)-nonenal — Oxidative stress.; Abbreviations: ADH, alcohol dehydrogenase; ALH, NADPH:2-alkenal α,β-hydrogenase; AKR, aldo-keto reductase; APX, ascorbate peroxidase; GC, gas chromatography; GST, glutathione S-transferase; HHE, 4-hydroxy-(2E)-hexenal; HNA, 4-hydroxynonylaldehyde; HNE, 4-hydroxy-(2E)-nonenal; LTB4DH, leukotriene B4 12-hydroxydehydrogenase; MS, mass spectrometry; P1-ZCr, P1-ζ-crystallin; PG, prostaglandin; PGR, 15-oxo-prostaglandin 13-reductase; ROS, reactive oxygen species; SOD, superoxide dismutase; ZCr, ζ-crystallin.

Journal Article.  7011 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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