Journal Article

ABA-Activated SnRK2 Protein Kinase is Required for Dehydration Stress Signaling in <i>Arabidopsis</i>

Riichiro Yoshida, Tokunori Hobo, Kazuya Ichimura, Tsuyoshi Mizoguchi, Fuminori Takahashi, Jose Aronso, Joseph R. Ecker and Kazuo Shinozaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 43, issue 12, pages 1473-1483
Published in print December 2002 | ISSN: 0032-0781
Published online December 2002 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcf188
ABA-Activated SnRK2 Protein Kinase is Required for Dehydration Stress Signaling in Arabidopsis

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Protein phosphorylation has pivotal roles in ABA and osmotic stress signaling in higher plants. Two protein phosphatase genes, ABI1 and ABI2, are known to regulate these signaling pathways in Arabidopsis. The identity of ABA-activated protein kinases required for the ABA signaling, however, remains to be elucidated. Here we demonstrate that two protein kinases, p44 and p42, were activated by ABA in Arabidopsis T87 cultured cells, and at least one protein kinase, p44, was activated not only by ABA but also by low humidity in Arabidopsis plants. Analysis of T-DNA knockout mutants and biochemical analysis using a specific antibody revealed that the p44 is encoded by a SnRK2-type protein kinase gene, SRK2E. The srk2e mutation resulted in a wilty phenotype mainly due to loss of stomatal closure in response to a rapid humidity decrease. ABA-inducible gene expression of rd22 and rd29B was suppressed in srk2e. These results show that SRK2E plays an important role in ABA signaling in response to water stress.

Keywords: Keywords: ABA — Arabidopsis — Protein kinase — Signal transduction — SnRK2.; Abbreviations: ABA, abscisic acid; GFP, green fluorescent protein; SnRK2, SNF-related protein kinase 2.

Journal Article.  6459 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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