Journal Article

Linear Dominance Relationship among Four Class-II <i>S</i> Haplotypes in Pollen is Determined by the Expression of <i>SP11</i> in <i>Brassica</i> Self-Incompatibility

Tomohiro Kakizaki, Yoshinobu Takada, Akiko Ito, Go Suzuki, Hiroshi Shiba, Seiji Takayama, Akira Isogai and Masao Watanabe

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 1, pages 70-75
Published in print January 2003 | ISSN: 0032-0781
Published online January 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg009
Linear Dominance Relationship among Four Class-II S Haplotypes in Pollen is Determined by the Expression of SP11 in Brassica Self-Incompatibility

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Self-incompatibility (SI) prevents self-fertilization by rejecting pollen from plants with the same S phenotype. The Brassica SI system is controlled sporophytically by multiple alleles at the single locus, S, and dominance relationships among S haplotypes are observed in both stigma and pollen. We have identified previously five different class-II S haplotypes in Brassica campestris. Here, we performed test-crosses between S heterozygotes and their respective parental S homozygotes for four of these class-II S haplotypes, and observed a linear dominance relationship on the pollen side. To determine how this relationship is controlled, we performed RNA gel blot analyses for six S heterozygotes and their respective parental S homozygotes using the corresponding SP11 clone as a probe. In all six S heterozygotes, SP11 derived from a dominant haplotype was predominantly expressed, and SP11 derived from a recessive haplotype was repressed. Thus, the linear dominance relationship of the SI phenotype on the pollen side is regulated by the expression of SP11.

Keywords: Keywords: Brassica campestris (syn. rapa) — Linear dominance relationship — Monoallelic expression — S multiple alleles — Self-incompatibility — SP11.; Abbreviations: MHC, major histocompatibility complex; NOR, nucleolus organizer region; SCR, S locus cysteine-rich protein; SI, self-incompatibility; SLG, S locus glycoprotein; SP11, S locus protein 11; SRK, S receptor kinase.

Journal Article.  3965 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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