Journal Article

<i>CvADH1</i>, a Member of Short-Chain Alcohol Dehydrogenase Family, is Inducible by Gibberellin and Sucrose in Developing Watermelon Seeds

Joonyul Kim, Hong-Gyu Kang, Sung-Hoon Jun, Jinwon Lee, Jieun Yim and Gynheung An

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 1, pages 85-92
Published in print January 2003 | ISSN: 0032-0781
Published online January 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg013
CvADH1, a Member of Short-Chain Alcohol Dehydrogenase Family, is Inducible by Gibberellin and Sucrose in Developing Watermelon Seeds

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To understand the molecular mechanisms that control seed formation, we selected a seed-preferential gene (CvADH1) from the ESTs of developing watermelon seeds. RNA blot analysis and in situ localization showed that CvADH1 was preferentially expressed in the nucellar tissue. The CvADH1 protein shared about 50% homology with short-chain alcohol dehydrogenase including ABA2 in Arabidopsis thaliana, stem secoisolariciresinol dehydrogenase in Forsythia intermedia, and 3β-hydroxysterol dehydrogenase in Digitalis lanata. We investigated gene-expression levels in seeds from both normally pollinated fruits and those made parthenocarpic via N-(2-chloro-4-pyridyl)-N′-phenylurea treatment, the latter of which lack zygotic tissues. Whereas the transcripts of CvADH1 rapidly started to accumulate from about the pre-heart stage in normal seeds, they were not detectable in the parthenocarpic seeds. Treating the parthenogenic fruit with GA3 strongly induced gene expression, up to the level accumulated in pollinated seeds. These results suggest that the CvADH1 gene is induced in maternal tissues by signals made in the zygotic tissues, and that gibberellin might be one of those signals. We also observed that CvADH1 expression was induced by sucrose in the parthenocarpic seeds. Therefore, we propose that the CvADH1 gene is inducible by gibberellin, and that sucrose plays an important role in the maternal tissues of watermelon during early seed development.

Keywords: Keywords: Developing seed — Gibberellin — Parthenocarpic fruit — Seed preferential — Sucrose-watermelon.; Accession number: CvADH1, AB018559; Cvrp15a, AB018560.

Journal Article.  5070 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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