Journal Article

Susceptibility of <i>Chara</i> Myosin to SH Reagents

Masaya Seki, Jun-ya Awata, Kiyo Shimada, Taku Kashiyama, Kohji Ito and Keiichi Yamamoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 2, pages 201-205
Published in print February 2003 | ISSN: 0032-0781
Published online February 2003 | e-ISSN: 1471-9053 | DOI:
Susceptibility of Chara Myosin to SH Reagents

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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Cellular and intracellular motile events in plants are susceptible to SH reagents such as N-ethylmaleimide (NEM). It has long been believed that the target of the reagent is myosin. We compared the effect of NEM on the motile and ATPase activities of skeletal muscle myosin with that on plant myosin using characean algal myosin. It was found that the motile activity of myosin prepared from NEM-treated C. corallina decreased to a level accountable for the decrease in the velocity of cytoplasmic streaming but it was also found that Chara myosin was far less susceptible to NEM than skeletal muscle myosin.

Keywords: Keywords: Chara corallina — Cytoplasmic streaming — Motility — Myosin — NEM — SH reagents.; Abbreviation: NEM, N-ethylmaleimide.

Journal Article.  3351 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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