Journal Article

Changes in α-Xylosidase during Intact and Auxin-Induced Growth of Pine Hypocotyls

María Sánchez, Cristina Gianzo, Javier Sampedro, Gloria Revilla and Ignacio Zarra

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 2, pages 132-138
Published in print February 2003 | ISSN: 0032-0781
Published online February 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg016
Changes in α-Xylosidase during Intact and Auxin-Induced Growth of Pine Hypocotyls

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A complete cDNA from Pinus pinaster Aiton, potentially coding for an α-xylosidase able to remove the xylose residue from xyloglucan oligosaccharides, has been cloned. Its sequence was homologous to previously published α-xylosidase genes from Arabidopsis and nasturtium. The protein also showed the two signature regions of family 31 of glycosyl hydrolases. The gene expression level was quantified by competitive RT-PCR, under different growth conditions, throughout seedling development, in different regions along the hypocotyls and in auxin-treated hypocotyl segments, and related with growth capacity and α-xylosidase activity. A role of α-xylosidase in regulating the level of xyloglucan oligosaccharides within the apoplast is proposed. The action of an α-xylosidase removing the xylose residue, would make possible the action of a β-glucosidase deblocking the xyloglucan oligosaccharide degradation and it could serve as a control point for the regulation of the apoplastic levels of xyloglucan oligosaccharides.

Keywords: Keywords: Auxin — Growth — Pinus pinaster — Xyloglucan — α-Xylosidase.; Abbreviation: XET, xyloglucan endotransglycosylase.

Journal Article.  4222 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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