Journal Article

Constitutive Expression of a Novel-Type Ammonium Transporter <i>OsAMT2</i> in Rice Plants

Arata Suenaga, Kanami Moriya, Yutaka Sonoda, Akira Ikeda, Nicolaus von Wirén, Toshihiko Hayakawa, Junji Yamaguchi and Tomoyuki Yamaya

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 2, pages 206-211
Published in print February 2003 | ISSN: 0032-0781
Published online February 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg017
Constitutive Expression of a Novel-Type Ammonium Transporter OsAMT2 in Rice Plants

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammonium-uptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.

Keywords: Keywords: Ammonium transporter — AMT2 — Antibody — Functional expression — Rice.; Abbreviations: AMT, ammonium transporter; HATS; high affinity transporter system; LATS, low affinity transporter system; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription-PCR.

Journal Article.  3386 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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