Journal Article

Characterization and Expression of a Novel Member (JBURE-II) of the Urease Gene Family from Jackbean [<i>Canavalia ensiformis</i> (L.) DC]

Melissa Pires-Alves, Maria Fátima Grossi-de-Sá, Grace B. S. Barcellos, Célia R. Carlini and Marcelo G. Moraes

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 2, pages 139-145
Published in print February 2003 | ISSN: 0032-0781
Published online February 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg018
Characterization and Expression of a Novel Member (JBURE-II) of the Urease Gene Family from Jackbean [Canavalia ensiformis (L.) DC]

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Canavalia ensiformis (jackbean) seeds contain the proteins urease and canatoxin, a variant form of the jackbean urease. Here we have cloned a cDNA encoding another isoform of urease, called JBURE-II. This cDNA was obtained by RT-PCR using as template total RNA extracted from C. ensiformis tissues. Nucleotide sequence analysis showed that JBURE-II clones share 86% similarity with known jackbean urease. The presence in C. ensiformis of a family of urease-related genes with at least three members was demonstrated by Southern blot analysis. In order to understand the pattern of expression of the JBURE-II gene, we collected tissue samples from different stages of flower and embryo development. The results of RT-PCR show that JBURE-II is expressed from flower buds throughout seed maturation. Semi-quantitative RT-PCR indicates that expression of urease and JBURE-II genes is induced in seedlings and in leaves treated with abscisic acid, a phytohormone involved in seed maturation and wound response. This work constitutes the first report on the presence of a family of urease genes in jackbean, and provides characterization of a cDNA encoding a new member of this gene family.

Keywords: Keywords: Abscisic acid — Canavalia ensiformis — Gene — Jackbean — Seed maturation — Urease (EC 3.5.1.5).; Abbreviations: ABA, abscisic acid; CNTX, canatoxin; DPA, days-post-anthesis; MS, Murashige and Skoog medium.

Journal Article.  3870 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.