Journal Article

An Abundant Periplasmic Protein of the Denitrifying Phototroph <i>Rhodobacter sphaeroides</i> f. sp. <i>denitrificans</i> is PstS, a Component of an ABC Phosphate Transport System

Masahiro Matsuzaki, Miho Abe, Satomi Hara, Yoshiko Iwasaki, Isamu Yamamoto and Toshio Satoh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 2, pages 212-216
Published in print February 2003 | ISSN: 0032-0781
Published online February 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg021
An Abundant Periplasmic Protein of the Denitrifying Phototroph Rhodobacter sphaeroides f. sp. denitrificans is PstS, a Component of an ABC Phosphate Transport System

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To understand a physiological role of an abundant 34-kDa periplasmic protein in the denitrifying phototroph Rhodobacter sphaeroides f. sp. denitrificans grown in a medium containing malate as the carbon source, the gene for the protein was isolated. The deduced amino acid sequence of the protein had a sequence similarity of 66.2% to that of PstS from Sinorhizobium meliloti. The downstream sequence of the Rhodobacter pstS contained five genes similar to pstCAB and phoUB, and its upstream sequence contained a putative regulatory sequence that is analogous to the Pho box involved in phosphate-limitation-induced gene expression in Escherichia coli. Both the amount of the PstS and the pstS promoter-driven expression of lacZ activity increased about two-fold in response to phosphate limitation. This is the first isolation of pst genes encoding proteins of an ABC phosphate transporter system from phototrophic bacteria.

Keywords: Keywords: Periplasmic protein — Pho regulon — Phosphate transport — Phototrophic bacteria — pst genes.; Abbreviations: ABC, ATP-binding cassette; DMSO, dimethyl sulfoxide; MBS, malate basal salt; PCR, polymerase chain reaction; Pit, phosphate inorganic transport; Pst, phosphate-specific transport.

Journal Article.  3929 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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