Journal Article

C-Terminal Domain of a Hevein-Like Protein from <i>Wasabia japonica</i> has Potent Antimicrobial Activity

Akinori Kiba, Hiromasa Saitoh, Masahiro Nishihara, Kaori Omiya and Saburo Yamamura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 3, pages 296-303
Published in print March 2003 | ISSN: 0032-0781
Published online March 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg035
C-Terminal Domain of a Hevein-Like Protein from Wasabia japonica has Potent Antimicrobial Activity

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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An antimicrobial protein, designated WjAMP-1, was purified from leaves of Wasabia japonica L. WjAMP-1 showed antimicrobial activity against both fungi and bacteria. The deduced amino acid sequence of cDNA of WjAMP-1 showed 60% and 70% identity with a hevein from Hevea brasiliansis and a hevein-like protein from Arabidopsis thaliana, respectively. However, matured WjAMP-1 lacked the hevein domain and may correspond to the C-terminal domain of hevein. Southern blot analysis showed that one or two copies of the WjAMP-1 gene were presented in the genome of wasabi. Expression of WjAMP-1 was detected in all organs tested, and was especially strong in petioles. Expression of WjAMP-1 was induced by the inoculation with fungal pathogens and treatment with methyl jasmonate. Recombinant WjAMP-1 expressed in Nicotiana benthamiana using potato virus X vector also inhibited not only growth of fungi but also bacteria. These results suggest that WjAMP-1 may be the C-terminal domain of hevein and one of the defense gene in W. japonica. WjAMP-1 gene may be useful genes to generate resistant plants against fungal and bacterial pathogens.

Keywords: Keywords: Antimicrobial protein — Hevein — Potato virus X vector — Wasabia japonica.; Nucleotide sequence reported in this paper has been submitted to DDBJ, EMBL and GenBank under the accession number AB091697.

Journal Article.  5335 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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