Journal Article

Cell Autonomous Circadian Waves of the APRR1/TOC1 Quintet in an Established Cell Line of <i>Arabidopsis thaliana</i>

Norihito Nakamichi, Akinori Matsushika, Takafumi Yamashino and Takeshi Mizuno

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 3, pages 360-365
Published in print March 2003 | ISSN: 0032-0781
Published online March 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg039
Cell Autonomous Circadian Waves of the APRR1/TOC1 Quintet in an Established Cell Line of Arabidopsis thaliana

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A small family of genes, named ARABIDOPSIS PSEUDO RESPONSE REGULATOR (APRR), are intriguing with special reference to circadian rhythms in plants, based on the fact that one of the members (APRR1) is identical to TOC1 (TIMING OF CAB EXPRESSION 1) that is believed to encode a clock component. In Arabidopsis plants, each transcript of the APRR1/TOC1 quintet genes starts accumulating after dawn rhythmically and one after another at intervals in the order of APRR9APRR7APRR5APRR3APRR1/TOC1. To characterize such intriguing circadian-associated events, we employed an established Arabidopsis cell line (named T87). When T87 cells were grown in an appropriate light and dark cycle, cell autonomous diurnal oscillations of the APRR1/TOC1 quintet genes were observed at the level of transcription, as seen in intact plants. After transfer to the conditions without any environmental time cues, particularly in constant dark, we further showed that free-running circadian rhythms persisted in the cultured cells, not only for the APRR1/TOC1 quintet genes, but also other typical circadian-controlled genes including CCA1 (CIRCADIAN CLOCK ASSOCIATED 1), LHY (LATE ELONGATED HYPOCOTYL) and CCR2 (COLD CIRCADIAN RHYTHM RNA BINDING 2). To our knowledge, this is the first indication of cell autonomous circadian rhythms in cultured cells in Arabidopsis thaliana, which will provide us with an alternative and advantageous means to characterize the plant biological clock.

Keywords: Keywords: Arabidopsis thaliana — Circadian rhythms — Cultured cells.; Abbreviations: APRR, ARABIDOPSIS PSEUDO RESPONSE REGULATOR; TOC1, TIMING OF CAB EXPRESSION 1; CCA1, CIRCADIAN CLOCK ASSOCIATED 1; CCR2, COLD CIRCADIAN RHYTHM RNA BINDING 2; DD, continuous darkness; LD, 12 h light/12 h dark; LL, continuous white light; LHY, LATE ELONGATED HYPOCOTYL.

Journal Article.  4370 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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