Journal Article

A Role of the –35 Element in the Initiation of Transcription at <i>psbA</i> Promoter in Tobacco Plastids

Keiko Hayashi, Takashi Shiina, Nao Ishii, Kayou Iwai, Yoko Ishizaki, Kazuya Morikawa and Yoshinori Toyoshima

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 3, pages 334-341
Published in print March 2003 | ISSN: 0032-0781
Published online March 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg041
A Role of the –35 Element in the Initiation of Transcription at psbA Promoter in Tobacco Plastids

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Most plastid promoters recognized by bacteria-like plastid RNA polymerase (PEP) are similar to E. coli σ70-type promoters comprising “–35” and “–10” elements. Among them, psbA promoter is unique in bearing additional elements between the conserved –35 and –10 elements. The psbA promoter activity is differentially maintained in the mature chloroplasts where the activity of most PEP promoters declines. Previously, we identified two types of PEP activities in wheat seedlings [Satoh et al. (1999) Plant J. 18: 407]; PEP present in the mature chloroplasts of the leaf tip (tip-type PEP) can initiate transcription from the –35-destructed psbA promoter, but the –35 element is essential for transcription by PEP present in immature chloroplasts of the leaf base (base-type PEP). To reveal which type of PEP functions in various types of plastids in tobacco, we analyzed the tobacco psbA promoter by means of a transplastomic approach. The promoter core context (–42 to +9) was sufficient for developmental regulation of the psbA promoter activity. The –35 promoter element was important for transcription initiation at the psbA promoter in all types of plastids, including chloroplasts in mature leaves, leucoplasts in roots, etioplasts in etiolated cotyledons. The conclusion is that the PEP bearing a promoter preference, similar to the wheat base-type PEP, functions dominantly in tobacco chloroplasts.

Keywords: Keywords: –35 element — Chloroplast transformation — GFP — Nicotiana tabacum — psbA promoter — Transcription.; Abbreviations: GFP, green fluorescent protein; MCS, multiple cloning sites; NEP, nuclear-encoded plastid RNA polymerase; PEP, plastid-encoded plastid RNA polymerase; SD, Shine–Dalgarno sequence; UTR, untranslated region.

Journal Article.  5540 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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