Journal Article

Temporal Association of Ca<sup>2+</sup>-Dependent Protein Kinase with Oil Bodies during Seed Development in <i>Santalum album</i> L.: Its Biochemical Characterization and Significance

Veena S. Anil, Alice C. Harmon and K. Sankara Rao

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 4, pages 367-376
Published in print April 2003 | ISSN: 0032-0781
Published online April 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg046
Temporal Association of Ca2+-Dependent Protein Kinase with Oil Bodies during Seed Development in Santalum album L.: Its Biochemical Characterization and Significance

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Calcium-dependent protein kinase (CDPK) is expressed in sandalwood (Santalum album L.) seeds under developmental regulation, and it is localized with spherical storage organelles in the endosperm [Anil et al. (2000) Plant Physiol. 122: 1035]. This study identifies these storage organelles as oil bodies. A 55 kDa protein associated with isolated oil bodies, showed Ca2+-dependent autophosphorylation and also cross-reacted with anti-soybean CDPK. The CDPK activity detected in the oil body-protein fraction was calmodulin-independent and sensitive to W7 (N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide) inhibition. Differences in Michaelis Menton kinetics, rate of histone phosphorylation and sensitivity to W7 inhibition between a soluble CDPK from embryos and the oil body-associated CDPK of endosperm suggest that these are tissue-specific isozymes. The association of CDPK with oil bodies of endosperm was found to show a temporal pattern during seed development. CDPK protein and activity, and the in vivo phosphorylation of Ser and Thr residues were detected strongly in the oil bodies of endosperm from maturing seed. Since oil body formation occurs during seed maturation, the observations indicate that CDPK and Ca2+ may have a regulatory role during oil accumulation/oil body biogenesis. The detection of CDPK-protein and activity in oil bodies of groundnut, sesame, cotton, sunflower, soybean and safflower suggests the ubiquity of the association of CDPKs with oil bodies.

Keywords: Keywords: Calcium-signaling — CDPK — Oil bodies — Santalum album L. — Seed development.; Abbreviations: CaM, calmodulin; CDPK, calcium-dependent protein kinase; D, dormant seeds; ER, endoplasmic reticulum; G, germinating seeds; MF, mature fruit; RF, ripe fruit; TAG, triacylglycerol; W7, N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide.

Journal Article.  6831 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.