Journal Article

Transformation of Tobacco with a Gene for the Thermophilic Acyl-Lipid Desaturase Enhances the Chilling Tolerance of Plants

Irina V. Orlova, Tatiana S. Serebriiskaya, Valeri Popov, Natalia Merkulova, Alexander M. Nosov, Tamara I. Trunova, Vladimir D. Tsydendambaev and Dmitry A. Los

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 4, pages 447-450
Published in print April 2003 | ISSN: 0032-0781
Published online April 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg047
Transformation of Tobacco with a Gene for the Thermophilic Acyl-Lipid Desaturase Enhances the Chilling Tolerance of Plants

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The desC gene for the acyl-lipid Δ9-desaturase from the thermophilic cyanobacterium Synechococcus vulcanus was introduced into Nicotiana tabacum under control of the 35S promoter. Expression of the desaturase was confirmed by Western blotting. Lipid analysis revealed that lipid content and the extent of fatty acid unsaturation significantly increased in leaves of transgenic plants. Chilling tolerance of those plants also increased, as estimated by the electrolyte leakage from the tissues damaged by cold treatments. Seeds of plants that expressed the desC gene imbibed at low temperatures demonstrated higher chilling tolerance than those of the control plants. The results demonstrate that the cyanobacterial thermophilic acyl-lipid desaturase was efficiently expressed in tobacco at ambient temperatures, and its expression resulted in the enhanced chilling tolerance of the transgenic plants.

Keywords: Keywords: Chilling tolerance — Fatty acid desaturase — Transgenic plants.

Journal Article.  2544 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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