Journal Article

The cDNA Microarray Analysis Using an <i>Arabidopsis pad3</i> Mutant Reveals the Expression Profiles and Classification of Genes Induced by <i>Alternaria brassicicola</i> Attack

Yoshihiro Narusaka, Mari Narusaka, Motoaki Seki, Junko Ishida, Maiko Nakashima, Asako Kamiya, Akiko Enju, Tetsuya Sakurai, Masakazu Satoh, Masatomo Kobayashi, Yukio Tosa, Pyoyun Park and Kazuo Shinozaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 4, pages 377-387
Published in print April 2003 | ISSN: 0032-0781
Published online April 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg050
The cDNA Microarray Analysis Using an Arabidopsis pad3 Mutant Reveals the Expression Profiles and Classification of Genes Induced by Alternaria brassicicola Attack

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The hypersensitive response (HR) was induced in a wild-type Arabidopsis thaliana plant (Columbia) (Col-wt) by inoculation with Alternaria brassicicola that causes the development of small brown necrotic lesions on the leaves. By contrast, pad3-1 mutants challenged with A. brassicicola produced spreading lesions. The cell death in pad3-1 mutants could not inhibit the pathogen growth and development, although both production of H2O2 and localized cell death were similar in Col-wt and pad3-1 plants after the inoculation. The difference between Col-wt and pad3-1 plants is defense responses after the occurrence of cell death. In other words, PAD3 is necessary for defense response to A. brassicicola. Therefore, we examined the changes in the expression patterns of ca. 7,000 genes by cDNA microarray analysis after inoculation with A. brassicicola. The cDNA microarrays were also done to analyze Arabidopsis responses after treatment with signal molecules, reactive oxygen species (ROS)-inducing compounds and UV-C. The results suggested that the pad3-1 mutation altered not only the accumulation of camalexin but also the timing of expression of many defense-related genes in response to the challenge with A. brassicicola. Furthermore, the plants integrate two or more signals that act together for promoting the induction of multiple defense pathways.

Keywords: Keywords: Alternaria brassicicola — Arabidopsis thaliana — cDNA microarray — Cell death — Hypersensitive response — Reactive oxygen species.; Abbreviations: dpi, day post-inoculation; ET, ethylene; hpi, hour post-inoculation; HR, hypersensitive response; JA, jasmonic acid; PR-protein, pathogenesis related- protein; ROS, reactive oxygen species; SA, salicylic acid; SAR, systemic acquired resistance.

Journal Article.  8220 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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