Journal Article

Immunohistochemical Studies on Translocation of Pollen <i>S</i>-haplotype Determinant in Self-incompatibility of <i>Brassica rapa</i>

Megumi Iwano, Hiroshi Shiba, Miyuki Funato, Hiroko Shimosato, Seiji Takayama and Akira Isogai

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 4, pages 428-436
Published in print April 2003 | ISSN: 0032-0781
Published online April 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg056
Immunohistochemical Studies on Translocation of Pollen S-haplotype Determinant in Self-incompatibility of Brassica rapa

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  • Biochemistry
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The self-incompatibility system in Brassica is controlled by the S-locus, which contains S-receptor kinase (SRK) and S-locus protein 11 (SP11). SRK and SP11 control stigma and pollen S-haplotype specificity, respectively. SP11 binding to SRK induces the autophosphorylation of SRK, which triggers the signaling cascade that results in the rejection of self-pollen. The localization of SP11 protein during pollen development and pollination, however, have never been demonstrated. In this study, we examined the localization of S8-SP11 protein in the anther or pollinated stigma by immuno-electron microscopy. The immunostaining suggested that S8-SP11 was secreted from the tapetal cell into the anther locule as a cluster and translocated to the pollen surface at the early developmental stage of the anther. During the pollination process, SP11 was translocated from the pollen surface to the papilla cell, and then penetrated the cuticle layer of the papilla cell to diffuse across the pectin cellulose layer. Furthermore, SP11 protein could only penetrate the cuticle layer of the papilla cell in the presence of pollen grains, and could not penetrate on its own. This suggests that another factor from the pollen grain is needed for SP11 protein to penetrate the papilla cell wall.

Keywords: Keywords: Anther — Brassica — Immunohistochemistry — Pollen S determinant — Pollination — Self-incompatibility.; Abbreviations: SCR, S-locus cysteine-rich protein; SEM, scanning electron microscopy; SI, self-incompatibility; SP11, S-locus protein 11; SRK, S-receptor kinase; TEM, transmission electron microscopy.

Journal Article.  5025 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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