Journal Article

Isolation of Stress-Related Genes of Rubber Particles and Latex in Fig Tree (<i>Ficus carica</i>) and their Expressions by Abiotic Stress or Plant Hormone Treatments

Jin Sun Kim, Yeon Ok Kim, Hyun Ju Ryu, Yeon Sig Kwak, Ji Yeon Lee and Hunseung Kang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 4, pages 412-414
Published in print April 2003 | ISSN: 0032-0781
Published online April 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg058
Isolation of Stress-Related Genes of Rubber Particles and Latex in Fig Tree (Ficus carica) and their Expressions by Abiotic Stress or Plant Hormone Treatments

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Two rubber particle protein genes and one latex gene in fig tree (Ficus carica) have been isolated and their expression following various abiotic stress treatments have been investigated. The two major proteins that are tightly associated with the catalytically active rubber particles have been sequenced to be peroxidase (POX) and trypsin inhibitor (TRI). A cDNA encoding a basic class I chitinase (CHI) has also been isolated from the fig tree latex. Wounding treatment strongly induced the expression of the three stress-related genes. Among the abiotic stresses investigated, drought treatment greatly induced the expression of POX, whereas the expression of CHI and TRI decreased after the same treatment. Cold treatment reduced slightly the transcript levels of the thee genes, and NaCl reduced marginally the expression of CHI. The expression of POX, CHI, and TRI was induced by jasmonic acid and abscisic acid, by jasmonic acid, and by salicylic acid, respectively. Different expression of the stress-related genes following various abiotic stress or plant hormone treatments suggests that a crosstalk exists between the signal transduction pathways elicited by abiotic stresses and hormones in plants. Our present results showing the expression of stress-related proteins on the surface of rubber particles and latex in F. carica also imply the possible role of rubber particles and latex in defense in rubber-producing plant species.

Keywords: Keywords: Chitinase — Ficus carica (fig tree) — Peroxidase — Rubber particle — Stress response — Trypsin inhibitor.; The nucleotide sequences reported in this paper have been submitted to the GenBank database under accession numbers AF479623 (peroxidase), AF479622 (trypsin inhibitor), and AY157614 (chitinase).

Journal Article.  5691 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.