Journal Article

Modulation of Pumpkin Glutathione <i>S</i>-Transferases by Aldehydes and Related Compounds

Masayuki Fujita and Mohammad Z. Hossain

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 5, pages 481-490
Published in print May 2003 | ISSN: 0032-0781
Published online May 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg060
Modulation of Pumpkin Glutathione S-Transferases by Aldehydes and Related Compounds

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Induction of pumpkin (Cucurbita maxima Duch.) glutathione S-transferase (GST, EC 2.5.1.18) by aldehydes and related compounds was examined. All of the tested compounds induced pumpkin GST to different degrees, and it was found that (1) aldehydes induce GST directly and alcohols induce GST indirectly, and (2) α,β-unsaturated aldehydes are the most effective inducers and their potency is related to the Michael acceptors reaction. The results of Western blot analysis showed that the patterns of induction of CmGSTU1, CmGSTU2 and CmGSTU3 were similar to the patterns of activity with the exception of α,β-unsaturated carbonyl compounds. Among the three compounds, crotonaldehyde caused the highest activity induction (9.2-fold), but Western blot expression was the highest only for CmGSTU3. CmGSTF1 was almost non-responsive to all of the tested stresses. Results of induction studies suggested that efficient pumpkin GST inducers have distinctive chemical features. The in vitro activity of the enzyme was inhibited by ethacryanic acid, trans-2-hexenal, crotonaldehyde, and pentanal. Ethacryanic acid was found to be the most potent inhibitor with an apparent I50 value of 6.90±2.06 µM, while others were weak to moderate inhibitors. The results presented here indicate that plant GSTs might be involved in the detoxification of physiologically and environmentally hazardous aldehydes/alcohols.

Keywords: Keywords: Aldehyde — Cucurbita maxima — Glutathione S-Transferase (GST) (EC 2.5.1.18) — Induction — Inhibition.; Abbreviations: GST, glutathione S-transferase; ADH, alcohol dehydrogenase; PDC, pyruvate decarboxylase.

Journal Article.  7727 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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