Journal Article

The <i>CaTin1</i> (<i>Capsicum annuum</i> TMV-induced Clone 1) and <i>CaTin1-2</i> Genes are Linked Head-to-Head and Share a Bidirectional Promoter

Ryoung Shin, Min Jung Kim and Kyung-Hee Paek

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 5, pages 549-554
Published in print May 2003 | ISSN: 0032-0781
Published online May 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg069
The CaTin1 (Capsicum annuum TMV-induced Clone 1) and CaTin1-2 Genes are Linked Head-to-Head and Share a Bidirectional Promoter

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  • Biochemistry
  • Molecular and Cell Biology
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CaTin1 was expressed relatively early in the TMV-inoculated leaves of hot pepper which is resistant to TMV-P0 infection. Interestingly, there was another homologous gene (CaTin1-2) located in front of CaTin1 in a head-to-head fashion and they shared a single promoter. The expression profile of the CaTin1-2 was very similar to CaTin1 in all the treatments except the slower induction time compared to CaTin1 upon TMV-P0 inoculation. The promoter analysis of CaTin1 and CaTin1-2 revealed bidirectionality both in cis-elements and activity. The CaTin1-2 promoter had two TATA-boxes, four GCC-boxes, the root responsive element, and a W1-box. The ethylene-inducible promoter activity depended on GCC-boxes and TMV-inducible activity of the CaTin1-2 promoter reached its highest activity when this promoter had a W1-box.

Keywords: Keywords: Bidirectional promoter — Ethylene — GCC-box — Hot pepper — TMV — W1-box.; Abbreviations: MeJA, methyl jasmonic acid; MV, methyl viologen; PR, pathogenesis-related; SA, salicylic acid; SAR, systemic acquired resistance; TMV, tobacco mosaic virus.

Journal Article.  3798 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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