Journal Article

Low-Temperature Induced Transmembrane Potential Changes in the Liverwort <i>Conocephalum conicum</i>

Elzbieta Krol, Halina Dziubinska and Kazimierz Trebacz

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 5, pages 527-533
Published in print May 2003 | ISSN: 0032-0781
Published online May 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg070
Low-Temperature Induced Transmembrane Potential Changes in the Liverwort Conocephalum conicum

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Intracellular microelectrode measurements revealed that the liverwort Conocephalum conicum generates all-or-none action potentials (APs) in response to a sudden temperature drop. In plants with anion and potassium conductance blocked, dose-dependent voltage transients (VTs) were evoked by cold stimuli. These VTs did not propagate. When the external concentration of Ca2+ was decreased or calcium channel inhibitors (La3+, Gd3+, verapamil, Mg2+, Mn2+) were used, inhibition of VTs was observed. Amplitudes of both APs and VTs grew when Sr2+ ions, known to release calcium from internal stores, were added to the medium. Neomycin, which suppresses phospholipase C and indirectly affects inositol triphosphate formation, caused substantial inhibition of both APs and VTs. It is concluded that a temperature drop elucidated membrane potential changes due to calcium influx both from external and internal stores.

Keywords: Keywords: Action potential — Calcium channels — Cold-induced potential changes — Liverwort Conocephalum conicum — Voltage transient.; Abbreviations: AP, action potential; A9C, anthracene-9-carboxylic acid; DC, direct current; DES, diethylstilbestrol; EGTA, ethylene glycol-bis-2-aminoethyl N,N,N′,N′-tetra acetic acid; IP3, inositol triphosphate; PLC, phospholipase C; RP, resting potential; TEA, tetraethyl ammonium; Vm, membrane voltage; VP, variation potential; VT, voltage transient.

Journal Article.  4835 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.