Journal Article

Enzymes of Jasmonate Biosynthesis Occur in Tomato Sieve Elements

Bettina Hause, Gerd Hause, Claudia Kutter, Otto Miersch and Claus Wasternack

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 6, pages 643-648
Published in print June 2003 | ISSN: 0032-0781
Published online June 2003 | e-ISSN: 1471-9053 | DOI:
Enzymes of Jasmonate Biosynthesis Occur in Tomato Sieve Elements

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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The allene oxide cyclase (AOC) is a plastid-located enzyme in the biosynthesis of the signaling compound jasmonic acid (JA). In tomato, AOC occurs specifically in ovules and vascular bundles [Hause et al. (2000) Plant J. 24; 113]. Immunocytological analysis of longitudinal sections of petioles and flower stalks revealed the occurrence of AOC in companion cells (CC) and sieve elements (SE). Electron microscopic analysis led to the conclusion that the AOC-containing structures of SE are plastids. AOC was not detected in SE of 35S::AOCantisense plants. The enzymes preceding AOC in JA biosynthesis, the allene oxide synthase (AOS) and the lipoxygenase, were also detected in SE. In situ hybridization showed that the SE are free of AOC-mRNA suggesting AOC protein traffic from CC to SE via plasmodesmata. A control by in situ hybridization of AOS mRNA coding for a protein with a size above the exclusion limit of plasmodesmata indicated mRNA in CC and SE. The data suggest that SE carry the capacity to form 12-oxo-phytodienoic acid, the unique precursor of JA. Together with preferential generation of JA in vascular bundles [Stenzel et al. (2003) Plant J. 33: 577], the data support a role of JA in systemic wound signaling.

Keywords: Keywords: Enzymes in JA biosynthesis — Immunocytochemistry — Sieve elements — Tomato.; Abbreviations: α-LeA, α-linolenic acid; AOC, allene oxide cyclase; AOS, allene oxide synthase; CC, companion cells; 13-HPOT, 13-hydroperoxy-octadecatrienoic acid; JA, jasmonic acid; JAME, JA methyl ester; 13-LOX, 13-lipoxygenase, OPDA, 12-oxo-phytodienoic acid; SE, sieve elements.

Journal Article.  4174 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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