Journal Article

Assessment of Utility of Meiosis-Associated Promoters of Lily for Induction of Germinal <i>Ds</i> Transposition in Transgenic Rice

Ryouhei Morita, Yoko Hattori, Shuji Yokoi, Hisabumi Takase, Masayoshi Minami, Kazuyuki Hiratsuka and Kinya Toriyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 6, pages 637-642
Published in print June 2003 | ISSN: 0032-0781
Published online June 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg074
Assessment of Utility of Meiosis-Associated Promoters of Lily for Induction of Germinal Ds Transposition in Transgenic Rice

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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In order to suppress the somatic excision of the Ds element and increase the independent transposition events of the Ac/Ds transposon tagging system in rice, we employed promoters of two meiosis-specific genes of lily, LIM10 and LIM18. The LIM10 promoter directed GUS expression specifically in anthers, with the LIM18 promoter doing the same in the anthers and somatic tissue. Both promoters induced independent germinal transposition with the frequency of approximately 1%. The LIM10 promoter, lacking induction of somatic transposition, is considered to be useful for improving transposon-tagging efficiencies in rice.

Keywords: Keywords: — Ds transposition — Meiosis-associated promoter — Oryza sativa L. — Transposon Ac/Ds.; Abbreviations: AcTP, Ac transposase; GUS, beta-glucuronidase, 35SP, cauliflower mosaic virus 35S promoter.

Journal Article.  3666 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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