Journal Article

Analysis of Sugars in Squash Xylem Sap

Hiroaki Iwai, Masako Usui, Haruko Hoshino, Hiroshi Kamada, Toshiro Matsunaga, Koichi Kakegawa, Tadashi Ishii and Shinobu Satoh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 6, pages 582-587
Published in print June 2003 | ISSN: 0032-0781
Published online June 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg075
Analysis of Sugars in Squash Xylem Sap

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Xylem sap contains organic and inorganic compounds that might be involved in root-to-shoot communication. To clarify the physiological functions of sugars in xylem sap, we characterized the sugar compounds of the xylem sap. The 80% ethanol-soluble fraction of xylem sap contained mainly myo-inositol and oligosaccharides. The 80% ethanol precipitate was solubilized with cyclohexanediamine tetraacetate and fractionated using anion exchange chromatography. The non-bound fraction from the anion-exchange column reacted with Yariv reagent and was rich in arabinogalactan, indicating the presence of arabinogalactan proteins (AGP). The bound fraction eluted with 50 mM ammonium formate buffer and separated using size exclusion chromatography producing the pectins rhamnogaracturonan (RG)-I and RG-II with apparent molecular masses of 15,000 and 11,000, respectively. These results indicate that the AGP, RG-I, borate cross-linked RG-II dimer and oligosaccharides produced by root tissues are transported to above-ground organs via xylem sap.

Keywords: Keywords: Arabinogalactan protein –– Boron –– myo-inositol –– Pectic polysaccharides –– Squash (Cucurbita) –– Xylem sap.; Abbreviations: AGP, arabinogalactan protein; Ara, arabinose; B, borate; CDTA, cyclohexanediamine tetraacetate; dRG-II-B, dimeric RG-II cross-linked borate ester; Fuc, fucose; Gal, galactose; GalA, galacturonic acid; GLC, gas-liquid chromatography; GlcA, glucuronic acid; ICP-MS, inductively coupled plasma-mass spectrometry; Man, mannose; mI, myo-inositol; T-Rha, terminal-linked rhamnose; RG, rhamnogalacturonan; SEC, size exclusion chromatography; Xyl, xylose.

Journal Article.  3612 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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