Journal Article

From Genome to Enzyme: Analysis of Key Glycolytic and Oxidative Pentose-Phosphate Pathway Enzymes in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803

Vicki L. Knowles and William C. Plaxton

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 7, pages 758-763
Published in print July 2003 | ISSN: 0032-0781
Published online July 2003 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcg086
From Genome to Enzyme: Analysis of Key Glycolytic and Oxidative Pentose-Phosphate Pathway Enzymes in the Cyanobacterium Synechocystis sp. PCC 6803

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Activities of glucokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucose isomerase, phosphofructokinase (PFK), enolase, pyruvate kinase (PK) and phosphoenolpyruvate (PEP) carboxylase were determined in extracts of photoautotrophic, mixotrophic, and heterotrophic cultures of Synechocystis sp. PCC 6803. Annotated genomes of Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120 were analyzed for the respective predicted physical properties of each enzyme investigated here. Enzymatic activity was largely unaffected by nutritional mode, with the exception of glucokinase and PK whose activities were significantly elevated in heterotrophic cultures of Synechocystis sp. PCC 6803. PFK activity was insensitive to bacterial PFK-A (allosteric) effectors such as PEP, implying that Synechocystis PFK should be classified as a PFK-B (non-allosteric). Immunoblot and kinetic studies indicated that irrespective of nutritional mode, the Synechocystis PK corresponds to a PK-A (AMP activated) rather than PK-F (fructose-1,6-bisphosphate activated).

Keywords: Keywords: Anabaena — Cyanobacteria — Genome sequence — Glycolysis — Oxidative pentose-phosphate pathway — Synechocystis.; Abbreviations: ENOL, enolase [EC 4.2.1.11]; F6P, fructose-6-phosphate; FBP, fructose-1,6-bisphosphate; GK, glucokinase [EC 2.7.1.2]; G6P, glucose-6-phosphate; G6PD, glucose-6-phosphate dehydrogenase [EC 1.1.1.49]; OPPP, oxidative pentose phosphate pathway; PEP, phosphoenolpyruvate; PEPC, phosphoenolpyruvate carboxylase [EC 4.1.1.31]; PFK, ATP-dependent phosphofructokinase [EC 2.7.1.11]; PFK-A and PFK-B, allosteric and non-allosteric isozymes of ATP-dependent phosphofructokinase, respectively; 6PGD, 6-phosphogluconate dehydrogenase [EC 1.1.1.44]; PGI, phosphoglucose isomerase [EC 5.3.1.9]; PK, pyruvate kinase [EC 2.7.1.40]; PK-A and PK-F, AMP- and fructose-1,6-bisphosphate-activated pyruvate kinase isozymes, respectively.

Journal Article.  3918 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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