Journal Article

An <i>Arabidopsis</i> MADS-Box Protein, AGL24, is Specifically Bound to and Phosphorylated by Meristematic Receptor-Like Kinase (MRLK)

Hidetomo Fujita, Miho Takemura, Emi Tani, Kyoko Nemoto, Akiho Yokota and Takayuki Kohchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 7, pages 735-742
Published in print July 2003 | ISSN: 0032-0781
Published online July 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg092
An Arabidopsis MADS-Box Protein, AGL24, is Specifically Bound to and Phosphorylated by Meristematic Receptor-Like Kinase (MRLK)

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  • Biochemistry
  • Molecular and Cell Biology
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Intercellular signaling mediated by receptor-like kinases (RLKs) is important for diverse processes in plant development, although downstream intracellular signaling pathways remain poorly understood. Proteins interacting directly with RLK were screened for by yeast two-hybrid assay with the kinase domain as bait. A MADS-box protein, AGL24 was identified as a candidate substrate of MRLK (Meristematic Receptor-Like Kinase), which was named for its spatial expression in shoot and root apical meristems in Arabidopsis. The AGL24 protein specifically interacted with, and was phosphorylated by, the MRLK kinase domain in in vitro assays. The simultaneous expression of AGL24 and MRLK in shoot apices during floral transition suggested that the interaction occurs in plants. Using plants constitutively expressing a fusion protein of AGL24 and green fluorescent protein, the subcellular localization of AGL24 protein was observed exclusively in the nucleus in apical tissues where MRLK was expressed, while AGL24 was localized in both the cytoplasm and the nucleus in tissues where no MRLK expression was detectable. These results suggest that MRLK signaling promotes translocation of AGL24 from the cytoplasm to the nucleus. We propose that the RLK signaling pathway involves phosphorylation of a MADS-box transcription factor.

Keywords: Keywords: Arabidopsis thaliana — MADS-box gene — Protein phosphorylation — Protein–protein interaction — Receptor-like kinase.; Abbreviations: DAS, day after sowing; GFP, green fluorescent protein; GST, glutathione S-transferase; KAPP, kinase-associated protein phosphatase; LRR, leucine-rich repeat; RLK, receptor-like kinase; RTK, receptor tyrosine kinase.

Journal Article.  6054 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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