Journal Article

Ectopic Expression of an Orchid (<i>Oncidium</i> Gower Ramsey) <i>AGL6</i>-like Gene Promotes Flowering by Activating Flowering Time Genes in <i>Arabidopsis thaliana</i>

Hsing-Fun Hsu, Chih-Hsiang Huang, Lu-Tung Chou and Chang-Hsien Yang

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 44, issue 8, pages 783-794
Published in print August 2003 | ISSN: 0032-0781
Published online August 2003 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcg099
Ectopic Expression of an Orchid (Oncidium Gower Ramsey)
AGL6-like Gene Promotes Flowering by Activating Flowering
Time Genes in Arabidopsis thaliana

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An AP1/AGL9 group of MADS box gene, OMADS1, with extensive homology to the Arabidopsis AGAMOUS-like 6 gene (AGL6) was characterized from orchid (Oncidium Gower Ramsey). OMADS1 mRNA was detected in apical meristem and in the lip and carpel of flower. Yeast two-hybrid analysis indicated that OMADS1 is able to strongly interact with OMADS3, a TM6-like protein that was involved in flower formation and floral initiation in orchid. Transgenic Arabidopsis and tobacco ectopically expressed OMADS1 showed similar novel phenotypes by significantly reducing plant size, flowering extremely early, and losing inflorescence indeterminacy. In addition, homeotic conversion of sepals into carpel-like structures and petals into staminoid structures were also observed in flowers of 35S::OMADS1 Arabidopsis. This result indicated that OMADS1 was involved in floral formation and initiation in transgenic plants. Further analysis indicated that the expression of flowering time genes FT, SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1) and flower meristem identity genes LEAFY (LFY), APETALA1 (AP1) was significantly up-regulated in 35S::OMADS1 transgenic Arabidopsis plants. Furthermore, ectopic expression of OMADS1 rescued late-flowering phenotype in gi-1, co-3 but not for ft-1 and fwa-1 mutants. These results supported that ectopic expression of OMADS1 influenced flower transition and formation by acting as an activator for FT and SOC1 in Arabidopsis.

Keywords: Keywords: Arabidopsis thaliana — Flower development — Flowering time — MADS box genes — Orchid — Oncidium Gower Ramsey.; Abbreviations: CaMV, cauliflower mosaic virus; CTAB, hexadecyl trimethyl-ammonium bromide; MS medium, Murashige and Skoog medium; RT, reverse transcription.

Journal Article.  8614 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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